Sentence examples for by sequential dissociation of from inspiring English sources

After vortexing and lysis of the red blood cells in NH4Cl, a single-cell suspension was obtained by sequential dissociation of the fragments by gentle pipetting for 1 2 min in 0.25% trypsin and then for 2 min in 5 mg/ml Dispase II plus 0.1 mg/ml DNase I, followed by filtration through a 40-mm mesh.

A suspension of single cells was obtained by sequential dissociation of the fragments by gentle pipetting and incubation at 37°C for 1 to 2 minutes in 0.25% trypsin.

After vortexing and lysis of the red blood cells in NH4Cl, a single cell suspension was obtained by sequential dissociation of the fragments by gentle pipetting for 1 2 minutes in 0.25% trypsin, and then 2 minutes in 5 mg/ml dispase II plus DNase I followed by filtration through a 40-mm mesh.

After vortexing and lysis of the red blood cells in NH4Cl, mammary epithelial cells were obtained by sequential dissociation of the fragments by gentle pipetting for 1 2 min in 0.25% trypsin, and 2 min in 5 mg/ml dispase plus 0.1 mg/ml DNase I (DNase; Sigma).

After vortexing and lysis of the red blood cells in NH4Cl, a single cell suspension was obtained by sequential dissociation of the fragments by gentle pipetting for 1-2 min in 0.25% trypsin, and then 2 min in 5 mg/ml dispase II plus 0.1 mg/ml DNase I (DNase; Sigma Aldrich, St . Louis MO, USA) followed by filtration through a 40-μm mesh (BD, Franklin Lakes, NJ, USA).

It was found that the sequential dissociations of CH4 were the dominant rate-determining process.

Highly enriched populations of rat testicular germ cells were prepared by sequential enzymatic dissociation of rat testis.

In order to improve the CO selectivity in CH4 chemical looping process for syngas production, we need to find promoters which could decrease the activation barrier of CH4 sequential dissociation steps and increase the oxygen migration ability of oxygen-carriers.

The enzyme is activated by sequential binding of product molecules with dissociation constant K P = (k P − / k P + ) for each binding step.

Briefly, neurons were obtained by sequential steps of enzymatic digestion and mechanical dissociation of DRGs harvested from P0 animals.

As is known from the literature sources (Kairbekov et al. 2008), the mechanism of the reaction involves a heterolytic dissociation of hydrogen into a metal cation, followed by sequential addition of the hydride ion and the proton on the double bond of hydrogenated compound.