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In this work, we also directly compared various heterologous two- or three-component vaccination regimens and found that the most potent at the vaccine doses used was a combination of the three employed vectors in a DNA priming followed by sequential boosts with rHAdV-5 and rMVA, or the DAM regimen.
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The adenoviral vectors were used to prime Balb/c mice followed by sequential boosting with chimpanzee type 63, and chimpanzee type 3 adenoviral vectors encoding SIVmac239 Gag and full length consensus Env.
Epitope-scaffolds were inoculated either in a homologous manner for each fusion protein (i.e., ES5 prime, followed by two ES5 boosts) or in a heterologous sequential manner (ES5, followed by ES1 and ES2 sequential boosts).
Dose escalation by a sequential boost regimen results in a longer treatment duration.
In the earlier dosimetric comparison [ 17] we showed that the best noninvasive technique to deliver a sequential boost was with the Vero SBRT system (joint product of BrainLAB; BrainLAB AG, Feldkirchen, Germany, and MHI; Mitsubishi Heavy Industries, Tokyo, Japan) [ 18].
We confirm these results when comparing SIB with a sequential boost, which has been performed with the best available technique according to an earlier comparison [ 17] and we show that advanced IMRT techniques are not required for SIB.
The ongoing RTOG 1005 trial will evaluate a hypofractionated dose schedule with SIB compared to a standard treatment schedule with sequential boost.
The dose to the organs at risk (OAR) was not influenced by SIB compared to sequential boost.
There was a statistically significant difference in the mean volume of the ipsilateral breast receiving 107% of the prescribed 50 Gy (Vol107) between the sequential boost and the SIB with CMS XIO (P = 0.001, Bonferroni post hoc analysis).
We present a dosimetric comparison of WBI with a sequential boost, compared to 3 different techniques of WBI with SIB.
A second explanation for better conformity can be the smaller margins around the PTVboost necessary for coverage with the SIB technique than with the sequential boost.
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