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The PCR was initiated by sequential amplification of 40 cycles at 95°C (15 s) and 60°C (60 s).
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One version of the [1 2] apm-2 entry vector was appended at the amino terminus with an HA tag (YPYDVPDYA) followed by a flexible linker (GTGGTGGSGGTG) by sequential amplification using primers oGH753-699 and oGH737-699 followed by recombination with the [1 2] donor to generate pGH445.
In SNP genotyping, TSP enables the sequential amplification of a target sequence harboring a polymorphism, followed by interrogation of the SNP in a single-step assay.
One example is in C. elegans, in which a gene expansion has occurred, evidently to aid in sequential amplification of siRNA signal.
Titrations were performed by sequential addition of ligand to enzyme.
Selection of successful recombinants is mediated by sequential expression of I-SceI endonuclease after recombination.
The modulation of muscle development processes is triggered by sequential events of gene activation and inhibition.
Sensitivity analysis was performed by sequential omission of individual studies.
by rapid amplification of cDNA ends (RACE).
Further confirmation was done by PCR amplification of ITS region.
But it certainly can feel that way, particularly when helped along by the amplification of increasingly histrionic media.
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