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OCR was measured by sequential additions of oligomycin A (Sigma), FCCP (Sigma) and antimycin A (Sigma).
Approximately 200 400 µg of protein cell lysates were incubated with 50 µL of Protein G/A beads (Miltenyi Biotec, Auburn, CA) and followed by sequential additions of 10 µL (2 µg) of cyclin D1 and Cdk 4 antibodies with end-to-end rotation overnight at 4°C.
Leak kinetics were measured by varying oxygen consumption and membrane potential by sequential additions of malonate (approx. 0.1 2.3 mM).
The biosynthetic pathway is mediated by sequential additions of sugars and other components to phosphatidylinositol [ 12– 12].
Leak kinetics were measured by varying membrane potential and oxygen consumption by sequential additions of malonate (approx. 0.1 2.3 mM).
Respiration was activated by adding 5 mM pyruvate and 5 mM malate (final concentrations) followed by sequential additions of 50 nmol ADP.
Similar(48)
Blunt-ended, double-stranded cDNA is obtained by sequential addition of enzymes to a single tube.
Each curve demonstrates fluorescence effects induced by sequential addition of 10 mM MgCl2, 16 μM RC24 and 25 mM EDTA.
Strand displacement cycles can be driven by sequential addition of short oligonucleotide sequences.
Fmoc and Boc-protected pseudo meso-tetraarylporphyrin-based amino-acids were synthesized by sequential addition of nucleophiles to cyanuric chloride.
Three series of polydiphenylsiloxane (PDPS -containing diblock coPDPS -containingnthesizediblockcopolymers molecular wereht and composynthesizedequential addition of the corresponding comonomers through a living anionic polymerization.
More suggestions(22)
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