Sentence examples for by sequential addition of from inspiring English sources

Blunt-ended, double-stranded cDNA is obtained by sequential addition of enzymes to a single tube.

Each curve demonstrates fluorescence effects induced by sequential addition of 10 mM MgCl2, 16 μM RC24 and 25 mM EDTA.

Strand displacement cycles can be driven by sequential addition of short oligonucleotide sequences.

Fmoc and Boc-protected pseudo meso-tetraarylporphyrin-based amino-acids were synthesized by sequential addition of nucleophiles to cyanuric chloride.

Three series of polydiphenylsiloxane (PDPS -containing diblock coPDPS -containingnthesizediblockcopolymers molecular wereht and composynthesizedequential addition of the corresponding comonomers through a living anionic polymerization.

Thus, aqueous suspensions of insoluble drugs have been subjected to powerful ultrasonic treatment followed by sequential addition of polycations and polyanions to the particle solution leading to assembly of ultra-thin polyelectrolyte shells on the nano-sized drug particles.

OCR was measured by sequential additions of oligomycin A (Sigma), FCCP (Sigma) and antimycin A (Sigma).

Approximately 200 400 µg of protein cell lysates were incubated with 50 µL of Protein G/A beads (Miltenyi Biotec, Auburn, CA) and followed by sequential additions of 10 µL (2 µg) of cyclin D1 and Cdk 4 antibodies with end-to-end rotation overnight at 4°C.

The biosynthetic pathway is mediated by sequential additions of sugars and other components to phosphatidylinositol [ 12– 12].

Leak kinetics were measured by varying oxygen consumption and membrane potential by sequential additions of malonate (approx. 0.1 2.3 mM).

Leak kinetics were measured by varying membrane potential and oxygen consumption by sequential additions of malonate (approx. 0.1 2.3 mM).