Digital images were obtained with a fluorescent microscope (Olympus BX51, Olympus, Melville, NY, USA) or by sequential acquisition.
Pulse wave velocity (SphygmoCor; AtCor Medical, Sydney, Australia) will be determined by sequential acquisition of pressure waveforms from the carotid and femoral arteries by use of a high-fidelity micromanometer (SPC-301; Millar Instruments, Houston, TX), as previously described.
Three-dimensional image stacks were acquired by sequential acquisition of views recorded along the z axis by varying the position of a piezo electrically controlled stage (typical step size of 0.8 μm).
High-magnification images were acquired along the z axis by sequential acquisitions of the FRET signal and the TMRM fluorescence to avoid cross talking between TMRM and YFP excitation.
Instead, the evolution of strains resistant to multiple antibiotics is driven by the sequential acquisition and accumulation of resistance-conferring mutations on the bacterial chromosome.
This sequential acquisition approach together with the relatively slow switching of the spatial phase randomizer do not lend themselves to rapid frame rates.
Confocal images were obtained using a Zeiss 510 confocal microscope (Carl Zeiss; a gift from F. Monzino) with a 63× objective (numerical aperture 1.4) with sequential acquisition settings of 1024 × 1024 pixels.
Confocal images were obtained with sequential acquisition settings at the maximal resolution of the microscope (1024×1024 pixels).
Confocal images stained neurons were obtained with sequential acquisition settings at the maximal resolution of the microscope (1024×1024 pixels).
Confocal images of transfected neurons were obtained with a LSM510 confocal microscope (Zeiss) using a Zeiss 63x (N.A. 1.4) oil objective with sequential acquisition settings of 1024×1024 pixels.
