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Entry clones for ChR2 and yfp were examined by sequencing to check that no errors were incorporated during the PCR amplifications.
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These sequences will have to be better studied by a longer -or complete- sequencing to check their identity, and then by verifying the expression level of the transcripts in different parts of the worm.
This means that clones need to be checked by sequencing to confirm their identity.
All constructs were checked by sequencing to exclude the presence of mutations.
Each time point was replicated three times from 2 independent biological samples, and all amplified cDNA fragments were sequenced by Beckman-Cogenics to check the specificity of the amplified products.
Then the sequences were inspected one by one to check whether it is real TE or to extract TE sequences from the position information provided in the descriptive text.
Consensus sequences were verified by BLAST searching to check cross-hybridization against each other and the AgMNPV-2D genome.
The sequences of all inserts were checked by sequencing.
Plasmids were checked by sequencing.
The construct was checked by sequencing.
The library diversity was checked by sequencing.
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