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Deletion breakpoints were established by sequencing the truncated PCR product.
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The promoter sequence was analysed by sequencing.
The insert sequences were confirmed by sequencing.
First, the truncated promoter region was subcloned into a previously reported cassette vector (Takeuchi et al., 1997) harboring a poly(A) signal, which was verified by sequencing.
DNA sequence of the truncated GH7 gene that was amplified and sequenced from R. miehei.
pEGFP-Ets-11 138, pEGFP-Ets-11 1380, pEGFP-Ets-11 13840, pEGFP-Ets-155 4400 and pEGFP-Ets-155 440ere pEGFP-Ets-155 440serting the truncated sequences of the Ets-1 coding region into the pEGFP-Ets-1136 440l II/KpEGFP-Ets-1136 440
These findings were further confirmed by subcloning the truncated WTX PCR products and subsequent didesoxy-sequencing.
This latter type was subsequently confirmed by sequencing to be a truncated version of the GI pseudogene.
ORF of the vegfr2 gene and a single 6.1-kb DNA fragment were confirmed by sequencing, indicating that the pCMV6-truncated-rVegfr2 plasmid vector had been constructed successfully.
and denote by the truncated power function.
Later, the method was performed by using automated sequencing machines, in which the truncated DNA molecules, labeled with fluorescent tags, were separated by size within thin glass capillaries and detected by laser excitation.
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