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The 32P-labeled RT products were fractionated on urea-acrylamide gels to determine their size in comparison to the bands obtained by sequencing the same mRNAs.
The absence of the BfaI site in undigested samples was confirmed by sequencing the same SRY fragment, which also indicated second banteng-specific mutation and differentiated between zebu and taurine origin [17].
In this paper, we performed a side-by-side comparison of stranded and non-stranded mRNA-seq by sequencing the same samples using both protocols.
Furthermore, this method can potentially discriminate polymerase chain reaction (PCR) errors from true sequences by sequencing the same DNA regions multiple times.
The relatively short length of the produced reads is compensated for by sequencing the same fragment from both ends; therefore, producing coupled reads separated by a 'gap', known as paired-end read.
We also retrieved "mate pairs" (i.e. the reads obtained by sequencing the same clone on the opposite direction) of recovered trace files to gain more information about the position of the two genes in the genome.
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To determine the taxonomic affinities of these strains, primers ITS5 and ITS4 [ 21] were used to amplify the approximately 540bp ITS, followed by sequencing with the same primers.
The PCR gaps were filled in using data from a hybridization enrichment experiment based on a Nimblegen custom array or solution pulldown (Supplementary Material), followed by sequencing in the same way.
Our goal is to place reads differing by sequencing errors into the same cluster, while placing reads corresponding to different antibodies into different clusters.
The SK-MEL-28 cell-line exon 15 T1796A (V599E) mutation was detected by sequencing, and the same mutation was also detected in two-thirds of the skin melanoma tumours studied.
In cases where bacterial DNA is detected, it can be further characterized by sequencing using the same primers.
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CEO of Professional Science Editing for Scientists @ prosciediting.com