Sentence examples for by sequencing the reverse from inspiring English sources

Sequencing was performed on the forward coding strand with confirmation of c-CBL alterations performed by sequencing the reverse strand as well.

The substitution of the P gene in the rescued viruses was confirmed by sequencing the reverse transcriptase-polymerase chain reaction (RT-PCR) products.

All mutations were confirmed by sequencing the reverse strand.

Mutations were identified in these samples by direct sequencing and were verified by sequencing the reverse strand or by HhaI digestion for Arg282 mutations.

All sequence instabilities detected in our set of samples were finally confirmed by sequencing the forward and reverse strains and replicated in a different laboratory by a different analyst.

The Illumina platform can also generate paired-end reads by sequencing the forward and reverse strands of each target DNA fragment.

Genotypic resistance to antiretroviral drugs was studied by sequencing the protease and reverse transcriptase genes with group M- or O-specific primers, depending on the serotyping results (8 ); samples that could not be typed with ELISA were tested with both group M and O primers.

When necessary, the result was reconfirmed by sequencing the other strand with the reverse primer.

In cells with restored sensitivity to Bsr and Neo, S3239A knockin was further verified by sequencing the corresponding DNA region amplified by reverse transcriptase-PCR (data not shown).

Confirmation of unreported base changes was performed by sequencing in the reverse direction on independent PCR products.

Sequences were trimmed and aligned manually in BioEdit (Hall 1999) and unique haplotypes were confirmed by sequencing in the reverse direction.