Sentence examples for by sequencing the plasmid from inspiring English sources

Colonies were selected after 2 days and the genes that contributed to this phenotype were identified by sequencing the plasmid DNA.

Insertions and/or deletions were easily determined by sequencing the plasmid DNAs recovered from the positive colonies.

Purify plasmid DNA from 2 to 3 independent, confirmed transformants using QIAprep Spin Miniprep Kit (Qiagen) as per product protocol and confirm the insert identity by sequencing the plasmid DNA with the 2235.F and/or 2615.R primer.

After confirmation by sequencing, the plasmid pMD19-Sl3-MMP was used for further experiments.

After checking the insert by sequencing, the plasmid was introduced into Saccharomyces cerevisiae strain W303-1A MATa [leu2-3112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} for expression analysis as follows.

Sequences of the probes representing genes with differential expression were obtained by sequencing the corresponding plasmid using the M13 forward or reverse primer.

Furthermore, generating the sequence of regions within captured gaps (e.g., during human-grade sequence finishing) is most readily achieved by sequencing the spanning plasmid subclones; in contrast, generating the sequence of regions within uncaptured gaps requires sequencing alternative templates (e.g., PCR products or purified BAC DNA), which is more costly and labor-intensive.

The correct recombination was confirmed by sequencing the obtained plasmids.

After verifying the clones by sequencing, the plasmids were purified and cut at one end using the appropriate restriction enzyme.

After both of the constructs were confirmed by sequencing, the plasmids expressing N-terminal His6-tagged BlaC WT and mutant proteins were transformed into Escherichia coli BL21/DE3 cells and cultured in LB broth at 37 °C.

The correct cDNA sequence and orientation were confirmed by sequencing the pGYT-HA-RASSF1C plasmid.