Genes of the CMY-positive isolates were identified by cycle sequencing the full-length amplified products with conditions and different primers as described (16 ).
We confirmed the in silico identified Gα-, Gβ- and Gγ-like genes from P. abies by cloning and sequencing the full-length coding sequences from cDNA libraries [KM197161 (PandA1) and KC825350.1-KC825354.1 (PaHGB1 – PaGG4)].
By sequencing the 11 full-length genes from 40 unrelated individuals encompassing nearly the entire natural range of P. tomentosa, we identified 2,114 SNPs in the c. 47,300 bp sequenced, with an average density of one SNP per 25 bp.
Out of the 38 SNPs genotyped over the Md-PG1 genomic region, 22 were identified by re-sequencing the full length (2395 bp) within the apple collection, with an average frequency of 1 SNP/108.9 bases.
The sequences of all mutant constructs were confirmed by sequencing of the full-length IP3R.
Species identification was accomplished by sequencing nearly the full length of the 16S rRNA gene, as previously described (5 ).
All clones were sequenced and the full-length sequence was constructed by the Chinese National Human Genome Center at Beijing.
By sequencing P. nigra full-length (PnFL) cDNA libraries, we generated about 116,000 5'-end or 3'-end ESTs corresponding to 19,841 nonredundant PnFL clones.
3 It was discovered in mice by sequencing random clones, and the full-length gene was proven to encode for a new tumor necrosis factor (TNF) receptor.
The identity of the European common mole, in which a novel hantavirus genome was detected, was verified by sequence analysis of the full-length 1,140-nucleotide 1,140-nucleotidee.
