Sentence examples for by sequencing the forward from inspiring English sources

All sequence instabilities detected in our set of samples were finally confirmed by sequencing the forward and reverse strains and replicated in a different laboratory by a different analyst.

The Illumina platform can also generate paired-end reads by sequencing the forward and reverse strands of each target DNA fragment.

Sequences of the probes representing genes with differential expression were obtained by sequencing the corresponding plasmid using the M13 forward or reverse primer.

The FcγRIIa-131H/R polymorphisms was analysed through a single PCR followed by sequencing using the forward primer 5′-CTGGTCAAGGTCACATTCTTC-3′ and the reverse 5′-CAATTTTGCTGCTATGGGC-3′, performing 35 cycles of denaturing at 94°C for 30 seconds, annealing at 50°C for 30 seconds, extension at 72°C for 30 seconds, and a final extension for 10 minutes at 72°C amplifying a 277 bp fragment [ 17].

Sequence variations, except well-known polymorphisms, were confirmed in an independent blood sample by sequencing both forward and reverse directions.

PCR products were gel purified and verified by sequencing using forward 5′-ttttcgttttaaaacctaagagtc-3′ and reverse 5′-tcatcggaagagagtagt-3′ primers.

Further validation was obtained by sequencing in forward and reverse directions from two DNA samples extracted on different occasions.

Detailed confirmation for each genetic group was performed by sequencing with forward and reverse outer or inner primers as needed.

All constructs were verified by sequencing using the CMV forward sequencing primer 5'CGC AAA TGG GCG GTA GGC GTG T 3' by the Vanderbilt Sequencing Core.

All constructs were verified by sequencing using the T7 forward and BGH reverse primers.

Mutations were confirmed by cloning PCR-amplified genes or gene fragments into the pCR4-TOPO cloning vector using the TOPO-TA Cloning Kit for Sequencing (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions, followed by sequencing with M13 forward and reverse primers.