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The molecular lesions of these revertants were identified by sequencing the corresponding coding regions, and they correspond to aminoacid substitutions (hppy1V2) and to premature stop codons (hppy4T1 and hppy2G1; see Fig. 1H).
Sequencing of the round 3 sub-library generated a greater percentage of location-collapsed HBV reads than were generated by sequencing the corresponding initial library (Table 1).
We verified the match of the G6pd2 primers by sequencing the corresponding parts of the G6pd2 gene in both Spr and B6 (data not shown).
Specific mutations can be analyzed by SNaPshot or by sequencing the corresponding exon.
The gag- pol boundary region was further confirmed by sequencing the corresponding cDNAs.
For variants identified in NTDs cases, we tested the cosegregation by sequencing the corresponding fragment in available additional family members.
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The identity of predictive genes was determined by partially sequencing the corresponding spotted cDNA clones and searching for gene similarities in public databases.
By sequencing the cDNA corresponding to the identified probes, we found mutations in the PARD3 and AS3 genes in the LNCaP and 22Rv1 cells respectively.
After determination of the precise exon-intron structure of each mGBP we confirmed the predicted sequences by cloning and sequencing the corresponding cDNAs out of IFNγ stimulated macrophages.
Haplotypes at DRO1 of 15 Japanese rice cultivars (Additional file 1: Figure S1) were determined by sequencing the genomic regions corresponding to the DRO1 transcribed regions as described previously (Uga et al. 2013a).
The final closure was confirmed by sequencing the PCR amplified corresponding contig-connecting fragments using the JL03 genomic DNA as the template.
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