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Co-author Professor Julian Parkhill, head of pathogen genomics at the Wellcome Trust Sanger Institute, said: "By sequencing the complete genomes of bacteria we can accurately describe where they have emerged from and how they pass from person to person.
All mutations were confirmed by sequencing the complete E6 ORF using flanking primers in the vector.
(iii) One early (May 2009) and one late (July 2009) viral isolates were characterized by sequencing the complete hemagglutinine and neuraminidase genes.
The classification of Venezuelan isolates as G2j was confirmed by phylogenetic analysis in the core and E1 E2 regions, and by sequencing the complete genome of one isolate.
By sequencing the complete coding region of the LIPH gene in 4 rex and 4 non rex rabbits, we identified a deletion of one nucleotide (1362delA) located in exon 9 and present in a homozygous state in the rex rabbit gene sequence (Figure 5A).
All mutations were confirmed by sequencing the complete open reading frame.
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The precise cassette exchange in both lines was then verified by amplifying and sequencing the complete PUb-DsRed marker cassette flanked by the heterospecific lox-sites.
The identity of babesial parasites from roe deer from Slovenia with EU1 was confirmed by cloning and sequencing the complete babesial 18S rRNA gene.
This was further confirmed by sequencing the PCR product which showed complete sequence identity with the published bovine non-specific AP sequence [ 22].
In conclusion, given that genotyping mtSNPs is straightforward compared with the intense effort demanded by sequencing complete genomes, the present study opens the door to more ambitious pan-African studies that would improve our knowledge on the mtDNA phylogeography in this continent.
The V207A and V207G mutations were identified by digestion with the FokI restriction enzyme and confirmed by sequencing of the complete coding sequence at The University of Iowa DNA Facility.
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