Sentence examples for by sequencing the coding from inspiring English sources
We determined the molecular basis of eight unrelated persons with Ko phenotypes by sequencing the coding and the intron-exon splice regions of KEL and, in some cases, analysis of mRNA transcripts and expression of mutants on the cell surface of transfected cells.
Snap-frozen biopsies from 109 patients collected prior to epirubicin (as preoperative therapy were investigated for TP53, CHEK2 and p14 ARF) mutations by sequencing the coding region and p14 ARF) promoter methylations.
Mutational analysis is carried out by sequencing the coding exons and flanking intronic regions of these two genes [ 19, 37, 84].
The identification of novel SNPs was accomplished by sequencing the coding regions of these genes in 96 apparently normal individuals (48 Caucasian Americans, 48 African Americans).
Its possible association with this condition was investigated by sequencing the coding exons of the FU gene in an affected member from the German family [ 17].
This gene was evaluated by sequencing the coding region and splice functions in normal and affected family members with familial atrial fibrillation.
Mutations were confirmed by DNA sequencing the coding region.
We studied the genetic variation in the WFDC locus by Sanger sequencing the coding regions of 18 WFDC and SEMG genes (comprising a total of 66 exons) and a number of intervening noncoding regions (spaced every ∼10 kb).
The C3.Dll1C413Y/+ mutant line was isolated from an ENU mutagenized combined sperm and genome archive by sequencing the Dll1 coding region [29].
No deletion, insertion or alternative splicing that could generate an inactive protein was detected by sequencing the whole coding sequence of the mRNA encoding the normal isoform 1 of the protein (not shown).
The molecular lesions of these revertants were identified by sequencing the corresponding coding regions, and they correspond to aminoacid substitutions (hppy1V2) and to premature stop codons (hppy4T1 and hppy2G1; see Fig. 1H).
