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Genotyping accuracy was confirmed by sequencing randomly selected samples.
The diversity of the library was confirmed by sequencing randomly selected clones.
The resulting library contained, on average, 2.2 amino acid mutations per protein, as determined by sequencing randomly selected acrB variants.
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The findings of the PCR-RFLP were confirmed by directly sequencing randomly selected samples, which was performed by Fuzhou Di'en Biotech Co., Ltd.
We further confirmed these deletions by cloning the deletion-specific PCR products and sequencing randomly selected clones.
By northern blot analysis, four sequences randomly selected from the 19 released sequences with no known function showed positive mRNA signals in epididymis and testis.
By sequencing 511 randomly selected cDNA clones, 72 of 293 ESTs could be assigned as fungal genes with sequence similarity to NCBI entries.
The quality of SSR enriched grasspea library was inspected by sequencing 186 randomly selected clones.
We validated our barFLEX collection by sequencing 54 randomly selected ORFs and 25 uptags.
A mutation frequency of one to five mutations per laccase gene was determined by sequencing ten randomly selected individual CotA clones.
We verified, by sequencing 10 randomly selected samples for each intron, that each variation observed was the one expected from databases.
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