Ten isolates from the antrum and corpus of stomach of each family member were analyzed both by sequencing of two housekeeping genes and macroarray tests.
The variant HIST1H2BN, not found at the protein level before this study, was identified by sequencing of two differentially modified C-terminal peptides from four subjects, as described above.
For instance, the H2AFZ variant, which is known to substitute for the canonical H2A histones and change the chromatin structure to affect the promoter regions of specific genes [37], [38], was found in two out of five subjects by sequencing of five differentially processed peptides corresponding to the N-terminus of this histone (Figure 2).
Bisulfite treatment, followed by sequencing of twelve individual clones from each of two FRDA and two control fibroblasts, did not show any alteration in methylation status at any of the nine CpG dinucleotides spanning the 5'UTR (including the two that overlap with the CTCF binding site), or in the first six CpG sites of exon 1.
Sequences were determined either by direct sequencing of the PCR fragment or by sequencing of two or three independent clones from different PCR reactions.
MST consisted of amplification followed by sequencing of ten different spacer regions of the C. burnetii genome: Cox2, 5, 18, 20, 22, 37, 51, 56, 57 and 61 [ 17].
Multilocus sequence typing (MLST) was performed on all W-135 isolates, as well as on stored isolates from the 2001 Hajj, as described by Maiden et al. (20 ) by sequencing of seven housekeeping genes.
Paired-end reads were generated by sequencing of four cloned insert libraries of 200, 700, 2,000, and 5,000 bp using Hiseq2000 system (Illumina Inc., San Diego, CA, USA) at BGI-Shenzhen, China.
Unlike the rice leaf and tricellular pollen tissues where the 21-nt small RNAs were the major reported small RNA population [ 28], filling or developing grains contained the most abundant 24-nt small RNAs by sequencing of ten small RNA libraries constructed from different stages of growing grains.
