By RT-PCR and sequencing of partial gene segment-2, PBVs in 15 of these 16 samples were assigned to genogroup-I.
HRV positive samples from hospitalized and control patients during September 1, 2004 through August 31, 2005 were further identified to species by RT-PCR and sequencing of partial region of the VP1 (D. Erdman, unpublished, method available on request).
Identification of flea species at the molecular level was achieved by PCR amplification and sequencing of partial siphonapteran 18S rDNA gene (1.95 kbp) as described (5 ).
Current detection and genotyping methods of genogroup (G) I and II noroviruses (NoVs) consist of a 2-step approach including detection of viral RNA by TaqMan realtime RT-PCR (RT-qPCR) followed by conventional RT-PCR and sequencing of partial regions of ORF1 or ORF2.
All positive fleas at screening were confirmed by using standard Bartonella PCR and sequencing of partial internal transcribed spacer gene fragments by using primers URBarto1 and URBarto2, as described (3 ).
Viral detection and identification were achieved by: PCR-amplification from fecal samples; sequencing of partial adenovirus polymerase (pol) and hexon genes; producing the virus in HeLa cells, with PCR and immunofluorescence detection, and with sequencing of the amplified pol and hexon gene fragments.
To identify the genomic constitution of this allohexaploid, four accessions of intermediate wheatgrass from its native area were analysed by sequencing of chloroplast trnL-F and partial nuclear GBSSI, and genomic in situ hybridization.
The data from this analysis show that it is possible to differentiate most mycobacterial species by sequence analysis of partial 16S rDNA.
The data from this analysis of all validly published mycobacterial species, in conjunction with the previously published evaluations of our database [ 33, 54], show that it is possible to differentiate most mycobacterial species by sequence analysis of partial 16S rDNA.
Pure isolates containing lipophilic inclusions were identified based on microscopy and by sequencing partial sequences of their 16SrRNA.
