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The use of PCR assays with degenerated primers, followed by sequencing has allowed the identification of several PV types in human and other animal hosts [ 15, 20].
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In conclusion, comparison of draft genomes obtained by next generation sequencing has allowed an in-depth study of diverse groups of bacterial spot pathogens at the genomic level.
High-throughput sequencing has allowed comprehensive discovery of somatic mutations in cancer samples.
The determination of complete genome sequences has allowed analysis by various statistical methods that have furthered understanding of the function of genomes.
The family specific complete sequences have allowed identification of post zygotic mutations between MZD genomes.
New sequencing technology has allowed for sequencing of DNA without the need for DNA amplification, generating sequencing from single molecules.
Second-generation sequencing technology has allowed a very large increase in sequencing throughput.
Comparison of RNA sequences bound by L22 has allowed for the establishment of a consensus L22 binding site consisting of a stem-loop structure with a G-C base pair at the base of the loop and a 5 7 nucleotide loop with a U residue at the 3' end [25].
Mapping of ESTs to an existing genome sequence by spliced alignment has allowed substantial refinement of gene models in a number of eukaryotic organisms (e.g. [ 17, 18]).
The sheer scale of sequence generated by these instruments has allowed unprecedented views into a number of molecular phenomena, including population genetics, transcriptomics, epigenetics and translational profiling.
However, the 16S rRNA gene sequence analysis technology has allowed bacterial evolution to be confirmed by experimental investigation, which has revolutionized in bacterial taxonomic history.
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