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B. ovis contains nine known deleted regions confirmed by sequencing as compared to B. melitensis [ 3].
In addition, there were discrepancies in expression pattern of some low abundant miRNAs during the infection detected by sequencing as compared to RT-PCR.
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Detection of both isoforms either in Sanger datasets (Phypa_199161) or in the 454 datasets (Phypa_74146 and Phypa_228333) could be explained by the higher sensitivity of sequencing as compared to the PCR-based approach or by the fact that adult gametophores were used to generate the 454 data, while the validation was carried out in the two principal tissues of the juvenile stage.
Go term enrichment analyses were conducted on i) transcripts significantly over transcribed in insecticide-selected strains compared to the susceptible strain, ii) transcripts significantly under transcribed in insecticide-selected strains compared to the susceptible strain and iii) transcripts affected by differential SNPs in their coding sequence as compared to the susceptible strain.
However, the prediction accuracy on independent dataset; representing a true unknown prediction showed that PSSM based model was able to predict 53 out of 54 sequences (as compared to 50 by hybrid-6 and HMM profiles of Pfam database).
Candidate genes for alternative splicing were identified by a significant read coverage within intron sequences as compared to the read coverage of the surrounding exons.
Disorder, which is encoded by a much wider portion of sequence space as compared to order, can indeed represent a strategy by which genes encoding overlapping reading frames can lessen evolutionary constraints imposed on their sequence by the overlap, allowing the encoded overlapping protein products to sample a wider sequence space without losing function.
Chromosomal integration of the TA modules limits their genetic mobility by horizontal gene transfer and may underlie the observation that chromosomal TA modules, which are mostly inherited by vertical descent, and have higher sequence conservation as compared to plasmid-borne modules.
Figure 7B illustrates the clustering patterns of sequences misclassified by geno2pheno as compared to those correctly classified.
Regions characterized by high recombination rates were enriched for specific GC-rich sequence motifs as compared to low recombinant regions.
Parameters α and β(=1− α) control the relative weight of sequence similarity (scaled by α) as compared to topological conservation (scaled by β) in the BP network alignment.
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