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Samples found to be positive for influenza A virus were screened for the H5 subtype, using a H5 specific RT-PCR, and, if negative, further subtyped by a RT-PCR for the 3'-part of the hemagglutinin (HA) gene, encompassing almost the entire HA2, and the full-length of the neuraminidase (NA) gene, followed by sequencing and characterization.
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Integrity of both constructs was verified by sequencing and phenotypic characterization.
Further characterization by sequencing and homology search indicated that this region is a part of the moaA3 gene which codes for molybdopterin cofactor protein A in M. bovis.
Further characterization by sequencing and homology search indicated that this region is a part of the moaA3 gene which encodes for molybdopterin cofactor protein A in M. bovis.
In this respect, we have found that use of dHPLC for single base mutations and MLPA for large genomic rearrangements is a reliable combination for use as an initial screening step followed by sequencing for characterization of the mutations identified.
Identity of the isolates was confirmed by 16 S rRNA gene sequencing and phenotypic characterization.
A bacterial strain which was marked as MB4 and identified by means of 16S rDNA sequencing and physiological characterization as either Bacillus pumilus or Bacillus altitudinis enhanced the extraction yield of Jatropha oil.
However, the majority of plant HATs have been identified by sequence characterization to other eukaryotes; thus, the functional characterization of the majority of these genes remains largely unknown.
Figure 1: Sequence analysis and characterization for the TRIC proteins.
Outbreaks associated with circulating VDPVs (cVDPVs) were first recognized by sequence characterization of isolates from polio cases in Hispaniola Haitii and the Dominican Republic) in 2000 2001 (97).
We have studied 101 MRSA with reduced vancomycin susceptibility from nine countries by multilocus sequence typing (MLST) and characterization of SCC mec (staphylococcal chromosomal cassette mec) and agr (accessory gene regulator).
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