The 96 samples were genotyped by sequencing, producing a total of 200,401,179 reads.
Mutants were identified by sequencing, and a total of 32 clones were selected for scFv purification and affinity characterisation.
The MEFV gene was analyzed in the 100 trios by direct sequencing, a total of 2.63 megabases of genomic sequence.
Simultaneously, 5 Gigabytes of Illumina data were released from the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, which were generated by sequencing the total RNA extractions from leaves, roots and seeds of Arachis hypogaea (Genotype: Baisha1016).
We demonstrated here that high numbers of genome-wide SNPs can be discovered by sequencing total genomic DNA of a complex genome with NGS platforms without a reference genome sequence.
By using degradome sequencing, a total of 15 targets were identified for 7 miRNA families, which reveal interaction between miRNAs and targets.
To identify novel genetic variants associated with cholelithiasis, the SLC10A2 gene was screened by sequencing, using a total of 93 samples from the Stuttgart population.
All constructs were checked by sequencing, and a total of 30 OSK genes and 9 F-box genes were cloned and sequenced.
A relatively high number of wDi transcripts (above 90%) were detected by sequencing total RNA from D. immitis AF and mf, while fewer transcripts were detected in the AM, L3 and L4 D. immitis samples (~50-60 ~50-60anscripts, Table 2).
PCR-RFLP was confirmed by repeated analysis, and genotyping was confirmed by sequencing 15% of total PCR products.
