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The validation of the 29 selected polymorphisms was carried out by sequencing a set of 32 DNA samples from control subjects.
We looked for the presence of polymorphisms with rare allele frequency ⩾5% in the P2RY5 gene (RefSeq NT_024524) in our population by sequencing a set of 48 genomic DNA samples from the UK breast cancer patients.
We conducted a characterization of the DNA methylation patterns of the S. moellendorffii genome by sequencing a set of S. moellendorffii shotgun genomic clones, along with a set of methylation filtered clones.
The aCGH results were validated by sequencing a set of mutation sites and the resolution of our array format was shown to be at the level of a single nucleotide mutation.
This study presents the results of a first investigation into the sequence conservation and putative function of D. pulex genes, which are identified by sequencing a set of 1,648 cDNA isolates that were interrogated in three microarray studies.
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Therefore, the entire gene pool can be fully sampled by sequencing a small set of representative isolates, and the number of new genes to be discovered by sequencing additional genomes will quickly approach zero.
A particularly efficient protocol, termed "restriction-site associated DNA" (RAD) [ 17], in combination with the Illumina Genome Analyzer sequencing device [ 18], discovers SNPs by sequencing a large set of restriction fragments [ 19- 21].
We determined the telomeres, subtelomeric regions, UTRs, coding regions and introns by sequencing a large set of macronuclear DNA sequences (4,242) and cDNAs (5,484) and comparing them with each other.
Those predicted SNPs shared among the three were then manually examined (Additional file 10) and then validated by sequencing a selected set of mutated genes (five in XI and six in XII) using gene-specific primer pairs (Additional file 12A).
We used genotyping by sequencing to identify a set of 10,480 single nucleotide polymorphism (SNP) markers for constructing a high-resolution genetic map of 1096 cM for watermelon.
To obtain the genomic location for genes of interest, referred to as "tagged genes", chromoWIZ provides several search methods (Table 1): By sequence homology a set of query sequences can be mapped against the annotated gene models using nucleotide or protein BLAST searches, requiring a predefined e-value and sequence identity.
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by sequencing a strain
by sequencing a total
by sequencing a panel
by sequencing a section
by sequencing a subset
by sequencing a region
by replacing a set
by sequencing a hemaglutinin
by sequencing a selection
by sequencing a reference
by sequencing a fraction
by sequencing a series
by sequencing a flaB
by sequencing a library
by sequencing a minimum
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