Sentence examples for by sequencing a reference from inspiring English sources
Several groups sidestep this problem by sequencing a reference genome alongside their target genome [11] [13].
The Mason read simulator (version 0.1.2) was used to simulate FASTQ files of next-generation sequencing reads that would be obtained by sequencing these reference genomes [ 36].
The number of repeat units in each fragment was determined by sequencing reference DNA samples with different alleles.
Because M. chelonae and M. abscessus have common phenotypic characteristics and not all samples were fully sequenced by a reference laboratory, we combined these for reporting as MABSC.
That meant that even understanding the genome by sequencing more than the reference genome was unlikely — until the technology changed.
Graduate student Nancy Chen, a population genetics fellow now at the University of California, Davis, started by sequencing the full genome of a reference scrub jay, and then assessed the genetic differences of all 3800 individual birds followed by the Florida group.
Selected bulks can now be genotyped en masse by high-throughput sequencing whenever a reference genome is available or can be obtained.
A total of 7,332 relevant nucleotide positions were analysed by APEX for the HIV PR region (78 nucleotides × 94 patient samples) and 4,752 for the HIV RT gene (99 nucleotides × 48 patient samples) and compared with the results obtained by standard Sanger sequencing as a reference method.
An important computational step is to determine the genes or coding sequences present, which is usually done by aligning the sequences against a reference database of protein sequences.
This strategy allows for easier elimination of sequence deviations caused by sequencing errors in the sequenced or the reference genomes.
Researchers at Perlegen Sciences, under contract to the National Toxicology Program, resequenced the genomic DNA of 15 genetically diverse research mouse strains by comparing the sequences to a reference mouse strain (the first mouse strain to have its DNA completely sequenced to very high accuracy).
