Sentence examples for by sequencing a minimum from inspiring English sources

Sequence analyses of the HA genes were performed by sequencing a minimum of 60 clones per sample that was calculated to detect with 95% probability any variant sequences at a level of 5% within the virus sample.

Pooling samples was expected to allow capture of a maximum of transcripts by sequencing a minimum of samples.

By sequencing at a minimum of 30× coverage, we have illustrated the higher sensitivity at detecting low-frequency and rare variants and the ability to investigate the presence of hotspots of functional mutations.

These BAC clones are being sequenced to a minimum of 4-fold sequence coverage.

The BAC clones were sequenced to a minimum six-fold coverage using Sanger sequencing methods on ABI 3730 xl by the company GATC, Germany.

This cDNA clone was subcloned and the sequence of human DOR was obtained by sequencing both strands with a two-fold coverage minimum.

On the other hand, the frequency of random sequencing errors on the Illumina GAIIx reduces efficiency by requiring a 20-40X average depth of sequencing and a minimum of 2-4X deep sequencing per DG marker during allele discovery and validation.

The HAltORF database (http://haltorf.roucoulab.com/) can be searched by gene name or symbol, by mRNA or protein GenBank accession number, and by protein sequence (with a minimum of 5 amino acids).

The "gold-standard" method of genome sequencing is characterized by developing a physical map followed by sequencing of the minimum tiling path.

In either case, a heuristic BAC by BAC sequencing approach from a minimum tiling path of the switchgrass genome should work.

All templates were sequenced for 76 cycles from both ends on the Illumina GAII platform, and reads merged into single fragments were included in subsequent analyses only if forward and reverse sequences overlapped by a minimum of 11 bp.