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Although cloning followed by sequencing a library of cloned fragments partially addresses this problem, this method has its own limitations and can introduce biases.
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Here we describe a large fraction of the transcribed genes by sequencing a cDNA library from the plasmodial stage of the developmental cycle.
We next explored the possibility of obtaining more continuity with GSFLX data by sequencing a "paired" library using the Roche/454 protocol (see Methods).
We tested MetaPORE by sequencing a nanopore library (Ebola2) constructed using the upgraded MAP-004 kit and corresponding to a whole blood sample from a patient with suspected Ebola hemorrhagic fever during the 2014 DRC outbreak.
A total of 1835 unigene ESTs were assembled by sequencing a normalized cDNA library, in which 500 SSR loci were identified.
Cui et al. (2010) developed 11 polymorphic microsatellite markers with a high polymorphism information content (PIC) by sequencing a microsatellite-enriched library.
By sequencing a small RNA library generated using pooled RNA from the pig heart, liver and thymus; we identified a total of 120 conserved miRNA homologs in pig.
Second-generation genome sequencing projects can benefit greatly by sequencing a diversity of libraries, which can reduce bias (Ross et al. 2013) and provide linking information at different scales (Gnerre et al. 2011; Birol et al. 2013; Wang et al. 2013).
In this study, we obtained 55,051 unique sequences by sequencing a non-normalized cDNA library from the cambial meristem and derivative cells of Japanese cedar.
We demonstrate the power of PBS by sequencing a pool of 32 human miRNA libraries on the SOLiD V2 platform (Applied Biosystems Inc .. 26 of these libraries were derived from human breast cancer cancerous tissues (BC) and 6 were derived from human breast noncancerous tissues (BO).
If a high cleavage and ligation efficiency (>80%) is achieved, bias toward sequencing only the last march steps of each amplicon can be counteracted by sequencing a pool of the marched sub-libraries from each round, rather than sequencing only the final round sub-library.
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