Sentence examples for by sequencing a flaB from inspiring English sources

Determination of the flagellin type of different isolates was performed by sequencing a flaB fragment (750 bp) amplified using the genus-specific set of primers BOR1 and BOR2 (Table 2).

Typing is performed by amplifying and sequencing a 750 bp fragment of the flaB gene delineated by primers BOR1 and BOR2 (Table 2).

Clonal descent among the H. pylori isolates infecting a family has been documented in one isolate from each biopsy of different members of families by comparing alleles of genes including vacA, flaA and flaB [12] and by sequencing three housekeeping genes (ureI, atpA and ahpC) [13].

Clonal descent has been demonstrated by comparing alleles of genes such as vacA, flaA, and flaB of isolates infecting members of the same family (10 – 12 ) and by sequencing three housekeeping genes (ureI, atpA, and ahpC) (13 ).

We confirmed identification of B. persica from the patient reported here by sequencing of both flaB and intergenic spacer domains (data not shown), as described (7, 8 ).

Sequencing of a flaB PCR amplicon (294 bp) indicated that the infectious borreliae was B. miyamotoi (GenBank accession no. AB921566) because the sequence was identical to that of B. miyamotoi HT31 (GenBank accession no. D43777).

The Borrelia-like organisms were identified as Borrelia sp. K64 by sequencing of PCR-amplified fragments of flaB and 16SrRNA genes from the cultured medium.

A BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi) search for the sequenced flaB gene (JX119098) demonstrated that the isolates were nearly identical with the type Achema strain of B. crocidurae (CP003426).

For confirmation of positive samples, a flaB gene nested PCR and sequencing of amplicons were performed as described (9 ).

Spirochetes were isolated from chronically infected mice, grown to late-log phase in BSK-H complete medium, harvested by centrifugation and analyzed for DbpA and DbpB synthesis by immunoblot analysis probed with a mixture of a FlaB mAb and anti-DbpA or -DbpB sera as described above.

Borrelial DNA was detected in a serum sample obtained on July 25 by 16S rRNA gene based-real time qPCR and a flaB gene based-realestimePCR.