A rye genome-specific DNA library was constructed by the deletion of sequences common to wheat and rye (Fig. 1).
An 89 bp segment of Revolver that is enriched in the genome of rye was isolated by deleting the DNA sequences common to rye and wheat.
A selection of samples found negative by the GPC PCR were subsequently retested by PCR targeted at a fragment of the nucleoprotein (N) gene (8 ), by using primers to sequences common to the Old World arenaviruses.
The identification of short sequences common to multiple genes by alignment algorithms can be hampered by the negative influence of non-common flanking sequences.
The template for transcription of the nuclease protection probe was created by cloning the sequence common to the expression vectors, from 100 bp upstream of the transcription start site to just 5' of the PTB ORF, followed by an inverted T7 promoter, into the Eco RI/Hinc II sites of the pUC19 vector (NEB).
In the experiment of Fig. 3, the six large plasmids were separated from chromosomal DNA by a 60 hrs-long agarose gel electrophoresis (Fig. 3A, track 1) and detected by hybridization with a sequence common to all 6 plasmidsprobeids (probe U, Table 3).
The best studied non-coding RNAs are microRNAs (miRNAs), 18 25 nt-long small RNA that epigenetically regulate translation by binding to a complementary "seed" sequence common to their "target" mRNA [2].
In this study, we investigate the functional associations of TNR sequences across different species to see whether cross-species analyses support the purported functional roles of repetitive sequences, and whether these functional roles can be explained by sequence properties common to multiple species.
The reverse sequences were first reverse-complemented and then concatenated with the corresponding forward sequence by mean of a sequence identifier common to the two mate pair sequences.
Because these individuals were unrelated and therefore only shared the SNPs measured by the array (and local common sequence polymorphisms in LD with these SNPs), these analyses highlighted a role for sequence polymorphisms common to the whole population in marking local differences in methylation propensity.
