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All the 57,313 unitranscripts were annotated by sequence similarity using BLASTx against the NCBI non-redundant (NR) protein database.
We first clustered transcripts from all 6 datasets by sequence similarity using CAP3 assembler [ 27].
To reduce the redundancy of our final set, we grouped the 356 sequences by sequence similarity using CD-Hit [ 54].
All contigs were taxonomically annotated by sequence similarity using BLASTN to a database containing 1411 reference genomes (extracted from NCBI database, June 2012 version).
Collembase can be explored library-specific using text queries (e.g. cluster name or BLAST annotation) and by sequence similarity using a local BLAST [ 33] server.
To remove redundancy and strong similarity of the proteins encoded by a RefSeq transcript, we used NCBI BLASTClust to cluster the proteins by sequence similarity using 80% coverage and 80% identity (parameters -L 0.8 -S 80).
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These tags were aligned into Taq I site associated tag piles by their sequence similarity using Stacks [ 75].
High-quality sequences were selected and bacteria were identified by searching for sequence similarity using BLAST (NCBI GenBank).
EuroPineDB can be browsed intuitively using several tabs, and data can be retrieved by text terms or sequence similarity using a stand-alone BLAST implementation.
Putative cellulosome-related regulatory elements were identified by BLAST searches and sequence similarity using known elements from C. thermocellum as queries [ 48- 50].
We identify Y-linked scaffolds by 1) searching for sequence similarity using known mammalian Y-linked gene sequences and 2) identifying scaffolds with sex-specific sequencing coverage characteristics indicative of Y linkage.
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