Sentence examples for by sequence optimisation and from inspiring English sources
Another set of 37 human proteins could be rescued by sequence optimisation and using linear templates for in vitro expression.
The first 20 cases (total 423) were used for MR sequence optimisation and for imager training.
We next examined the effect of sequence optimisation on protein expression rate and protein yield by selecting randomly 87 out of the 960 ORFs where protein expression had been unsuccessful in vivo or where transformation had failed in BL21 DE3 pLysS.
After sequence optimisation for the E. coli in vitro system and generating linear templates for wheat germ expression, the success rate of cell-free protein expression reached 93%.
More recently, several membrane protein structures have been obtained thanks to sequence optimisation using this method.
These sequence optimisations reduce unfavourable secondary structures in mRNA and thus improve the success rate of translation and protein expression.
The expression level was improved by codon optimisation and translated to a 77%and 101% increase in glycosylation efficiency (using Western blots and pSRM, respectively).
The three main tasks performed by HOMER are simulation, optimisation and sensitivity analysis [13].
In order to solve the sequence of optimisation problems, fmincon optimisation function from the MATLAB optimisation toolbox adapted to multiple output analysis of FE models is developed.
Crucial considerations in assessing the performance of modularity optimisation approaches are: (i) the scale and optimality handled by modularity optimisation methods and (ii) the resolution limit problem for small-size modules in large networks.
