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The gene encoding Sp17 is mapped by sequence database to chromosome 11 in human.
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RNA interference was designed by searching the public available sequence database to find a 19 bp oligonucleotide sequence specific for SAMD9 and inserting into a pSUPER-RNAi-EGFP expression vector to generate the SAMD9 RNA interference vector (pSUPER-RNAi-SAMD9).
It is the latter strategy that holds great potential for the future by screening of sequence databases to identify genes of interest, after which the targeted genes are codon-optimized and synthesized before being expressed in the target host.
The latter analysis yields amino acid sequences by which the original proteins can be identified, again by sequence database comparison.
Furthermore, we have already identified some putative meso- DAPDH genes in hyperthermophilic bacteria like Thermotoga species by sequence database, and the functional analyses of meso- DAPDH gene homolog in Thermotoga species are now under investigation.
All MS/MS data was analyzed by sequence database searching using the program Sequest [86] against protein sequences derived from all known open reading frames in S. cerevisiae.
Bacterial genomes containing rtx genes, as identified by sequence database screening.
After the peptide mixture is separated using a reverse-phase liquid chromatography electrospray ionization mass spectrometer, the peptides are identified by sequence database analysis [ 16].
However, a large number of unclassified and uncultured genera were also shown by sequence databases, the relations between CP and gut microbiota composition and how gut microbiota utilize IOP under oxidant injury need further research.
A gene product is the most common object of annotation, and all such objects require a stable identifier such as those specified by sequence databases maintained by European Bioinformatic Institute (EBI) and National Center for Biotechnology Information (NCBI).
Bionemo has been developed by manually associating sequence database entries to biodegradation reactions based on the information extracted from published articles [ 13].
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