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For all four BACs, we specifically tested how well the gene space was covered by sequence contigs from experiment 2. Here, we defined gene space as the coding region plus 1.5 kb upstream and 1 kb downstream.
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To address this need, we have constructed a macaque-specific high-density oligonucleotide microarray by using highly fragmented low-pass sequence contigs from the rhesus genome project together with the detailed sequence and exon structure of the human genome.
AGORA works by first aligning long sequence contigs extracted from de Bruijn graph edges to the consensus optical map.
The sequence contigs assembled from the 6B survey sequencing were mapped to the corresponding BAC contigs using their sequence similarities with the WGP tag sequences by BLAST.
Repeats such as LTRs or entire multicopy transposons were very poorly covered by the assembled sequence contigs of all four BACs because sequences from different copies of repeats were pooled and assembled into "consensus contigs".
Since multiple contigs could result from the same gene owing to either alternative splicing or from errors during sequencing and assembly, we attempted to remove the redundancy that arose from errors by clustering the sequence contigs.
In P. infestans, the region did not assemble continuously and is represented by segments from three different sequence contigs.
The 94 sequence contigs provided by 454 Life Sciences Corp (454 sequence contigs).
Sequence contigs were assembled by aligning the AB1 files against GenBank reference sequence files that were obtained from the National Center for Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov/Genbank/).nih.gov/Genbank/
A total of 448 unique sequences were obtained from an assembly of 535 sequences by Sequencher V4.2 (http://www.genecodes.com/), including 46 sequence contigs (Table 1) and 402 sequence singletons.
Gaps between ordered clones or sequence contigs are the second type, which is easily closed by sequencing of bridging clones obtained from pair-end sequence data.
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