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Rodent species were determined by sequence comparisons using the BLAST algorithm (www.ncbi.nlm.nih.gov).nih.gov
The identity of these sequences was ascertained by sequence comparisons using the program BlastN of the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/).nih.gov/
Annotation of COG and KEGG pathways was performed by sequence comparisons using BLASTX algorithm against Cluster of Orthologous Groups database and Kyoto Encyclopedia of Genes and Genomes database with an E-value threshold of 10-5.
The identity of these sequences was ascertained by sequence comparisons using the program BLAST Basic Local Alignment Tooll) of the National Center for Biotechnology Information (http://blast.ncbi.nlm.nih.gov/) By IFAT, four cats/samples tested positive for Giardia duodenalis cysts and one cat for Cryptosporidium sp. oocysts.
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The analysis of sequence redundancy was performed by sequence comparison using local BLASTN through a clustering system running under an alpha version of Biopipeline® and by using the Cap3 contig assembly program [ 22].
Markers not showing size polymorphisms were further analyzed for species-specific RFLPs by sequence comparison using Sequencher software (Gene Codes Corporation), the TCAG program available as part of the Biology Workbench software utilities provided at the San Diego Supercomputer Center [ 37], or with the SNP-RFLPing program [ 38].
Additionally, we validated inferences of absence from the OrthoMCL analyses by performing detailed sequence comparisons using BLAST+ [ 53] (Additional file 2).
AAI is calculated based on conserved protein-coding genes between a pair of genomes which are determined by whole-genome pairwise sequence comparisons using the BLAST algorithm [ 34].
Distances between a pair of genomes are determined by whole-genome pairwise sequence comparisons using one out of six supported local-alignment programs available in the GGDC.
Genes conserved between a pair of genomes were determined by whole-genome pairwise sequence comparisons using the BLAST algorithm release 2.2.5 [ 47].
If a 15 base match was subsequently identified, it was followed by local, 7 bp sequence comparisons using a 400 bp fragment generated from 200 bp up- and downstream sequences.
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