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Primer efficiencies were > 90%, and specificity was confirmed by sequence blast and melting curve analysis.
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Each selected peptide was confirmed as a unique CD4 peptide by sequence blast against human non-redundant genome database (NCBI).
Two additional, recently-identified, human complex I assembly factors, ECSIT and C20orf7, were found to have C. elegans homologues by protein sequence blast (www.wormbase.org) [38], [39].
Tools available at these three databases include options to search for sequences by BLAST and by homolog descriptions or Gene Ontology terms.
AKAP7 splice variant exon positions and splice junctions were verified by using NCBI two-sequence BLAST (bl2seq) and manually aligning cDNA sequences to the genomic sequences using Mega v5 [ 59].
Positive detection was verified by sequencing [17] and BLAST analysis [18].
MytiBase (http://mussel.cribi.unipd.it/) contains clusters of Mytilus galloprovincialis ESTs with consensus sequences annotated by Blast and InterProScan.
Phylogenetic distribution of PTL sequences obtained by BLAST and PSI-BLAST searches was restricted to pathogenic bacterial species (supplementary table S1 in supplementary file S2, Supplementary Material online) (fig. 2 A).
Targeted loci were identified by sequence analysis blasting the medaka genome (Assembly HdrR, Version 70.1).
Each chromosome was scanned by eye for duplicate genes positioned in tandem; where the similarity was not obvious from annotation suspected duplicates were tested by reciprocal BLAST and sequence alignment.
The nucleotide sequences and deduced protein sequences were analyzed by BLAST and ClustalW programs of the European Bioinformatics Institute (http://www.ebi.ac.uk/) [ 13].
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