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HAV cDNA (confirmed by sequence analysis) was cloned into a TOPO vector (Invitrogen, Carlsbad, CA), and four primer sets were designed for application in SYBR Green real-time RT-PCR.
Presumably, the lack of identification of some cellulases by sequence analysis was a result of low sequencing depth of the fosmid library reads for particular clones in the pooled library format.
A lineage related antibody group defined by sequence analysis was further confirmed by their cross reactivity to human and murine antigens, and exchangeability of heavy and light chains among the antibodies.
Placing the treeshrew on the mammalian tree by sequence analysis was somewhat problematic.
A further distinction highlighted by sequence analysis was that a second allele of estB was present in a separate scaffold within the O157 SvETEC genome.
The critical sensory region identified by sequence analysis was comparable in spite of the two different organisms and different proteins (for NARQ_ECOLI periplasmic region: position 35 146; numbering according to the E. coli Uniprot sequences).
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Interestingly, the novel extra base-pairing sequences, identified by sequence analysis, were also contacted by Nop1.
The other strains investigated by sequence analysis were unrelated to those from these familial groups.
In particular, the confirmation of the high frequency of unusual G/P combinations found by sequence analysis is recommended.
Our analyses suggest that both the S. cereviciae Trs130 (by membrane-extraction analysis) and the human TMEM1 (by sequence analysis) are not trans-membrane proteins.
Clones containing FMDV and IAV epitope sequences were selected and after being confirmed by sequence analysis, were expressed in E. coli and analyzed for protein expression.
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