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Genotyping the TOE1 g.2171G>A SNP was performed by sequence analysis using the automated sequencer Genetic Analyzer 3500 (Genetic Analyzer 3500, Applied Biosystems by Life Technologies GmbH, Darmstadt, Germany).
The amino acid sequences of the synthetic peptides were confirmed by sequence analysis using a protein sequencer PPSQ-21A (Shimadzu, Kyoto, Japan), and mass spectrometry using an AXIMA-CFR plus mass spectrometer (Shimadzu).
A total of 19 discordant autosomal genotyping results were obtained that were examined by sequence analysis using Roche-454 next generation sequencing and/or Sanger sequencing.
The genetic composition of the viral quasi-species present in the nasal washes of ferrets after 10 passages of A/H5N1wildtype and A/H5N1HA Q222L,G224S PB2 E627K was determined by sequence analysis using the 454/Roche GS-FLX sequencing platform (Roche, Woerden, the Netherlands) (tables S1 and S2).
The Mamu-DRB allele of individual macaques was determined by sequence analysis using the Genbank data base [15], [20].
The deletion junction sequences of all samples were further analyzed by PCR amplification across the deletion junction, followed by sequence analysis using the BigDye Terminator Cycle Sequencing kit (version 3.1) and the ABI3730XL automated DNA sequencer with Mutation Surveyor V3.20 software (Softgenetics, State College, CA).
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The library construction and size separation was performed at the Norwegian Genomics Consortium's genomics core facility followed by sequencing analysis using Illumina's Genome Analyzer II × sequencing platform.
The PCR products of transgenic FasL cDNA were then purified from the gel using the QIAquick Gel Extraction Kit (QIAGEN, Valencia, CA, USA) and were confirmed by sequencing analysis using BigDye Terminator Ready Reaction Kits (PE Biosystems, Foster City, CA, USA).
Sequencing of primer and probe binding sites was performed on all prenatal specimens, and blood specimens with an SMN1 copy number of <2, by bidirectional sequence analysis using BigDye Terminator Cycle Sequencing Kit (version 3.1) followed by capillary electrophoresis (Applied Biosystems, Carlsbad, CA, USA).
In such cases, since October 2012, the test kit material has been used directly for identification to a species level by DNA sequence analysis using species-specific primers (McDougal 2012; publication in preparation).
In addition, we also found three other potential NFAT binding sites, N3, N4, and N5, by computer sequence analysis using Genomatrix software.
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by immunoblot analysis using
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