In selected tissue cases, EGFR exon 19 uncommon mutation were also confirmed by di-deoxy Sanger direct sequencing analysis, supported by Eurofins MWG Operon service, (Ebersberg, Germany), after DNA amplification.
The fidelity of the rescue system was further supported by sequence analysis of RT-PCR derived amplicons corresponding to the VP1 coding sequence obtained directly from the initial clinical samples and also following virus rescue within cultured cells.
Our results were supported by sequence analysis of the 3' UTR regions of predicted target genes, and we found that the previously reported conclusion that a large fraction of the proteome is regulated by microRNAs was not supported by our statistical analysis of the data.
This conclusion is further supported by sequence analysis of 2 protein-coding genes, sodB and groEL.
This result was supported by sequence analysis of the selected BAC clones.
This is supported by sequence analysis of other proteins involved in glycogen production.
This model is supported by sequence analysis which suggests that GGA4p retains the ancestral sequence characteristics of the smaller chromosome it once was i.e. high gene density, compact genes, high CpG-island density, high recombination frequency and GC-richness [ 1] whereas GGA2 does not.
A possible explanation could be that this patient was infected in the early epidemic phase, which is supported by sequence analysis; this isolate has 29 extra nucleotides between ORF8a and ORF8b, a feature shared among isolates from civets and early phase patients.
Successful germline transmission was confirmed by genomic PCR (see Figure S1C in Supporting Information) and by sequence analysis (not shown).
These were verified by sequence analysis.
Correct assembly of each TALE construct was verified by sequence analysis (Supporting Information Table S1).
