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Most TDF sequences were annotated by sequence alignments using BLAST searches against NCBI databases.
The size and genomic positions for the different LmjSIDER2 or LbSIDER2 elements were determined by sequence alignments using ClustalW and manual corrections.
Multiple sequence alignments were performed using Clustal W (Kyoto University, Bioinformatics Center; http://www.genome.jp/tools/clustalw/). Phylogenetic relationships between the CNS strains were performed by sequence alignments using the Clustal X 2.0 software package [ 24].
Vector and bacterial contaminated DNA sequences were identified by sequence alignments using megaBLAST to all UniVec and non-redundant bacterial sequences from NCBI respectively, and hits with 95% identity were subsequently masked with N's.
All the catfish genome contigs involved in potential duplications were retrieved and visually checked by sequence alignments using BLASTN at a cutoff value of 1E-10 and minimum alignment length of 100 bp.
Also removed were sequences representing bacterial, yeast or fungal contaminations identified by sequence alignments using BLAST [ 62, 63] to E. coli K12 DNA sequence (GI: 6626251), Saccharomyces cerevisiae (GenBank, ), Aspergillus nidulans (TIGR ANGI.060302), and Agrobacterium tumefaciens (custom database generated using SRS, Lion Biosciences).
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The extension consists of an additional top node in the HTM topology that stores and compares sequences of input data by sequence alignment using dynamic programming.
Pairwise nucleotide polymorphisms among four strains (K7, S288C, YJM789 and EC1118) were analyzed by sequence alignment using MUMmer 3.0 software.
DOGMA annotations were manually checked by blast searches with orthologous sequences from other Cupressaceae plastomes, and, in some cases, by sequence alignment using MUSCLE version 3.8.31 (Edgar 2004).
This allows the user to retrieve proteins from Cildb, either in the whole database or for a given species, by sequence alignment using the NCBI BLAST algorithm.
Canonical (-10 and -35) and Fnr promoter sequences and Shine-Dalgarno sequences were searched within 130 nucleotides upstream to the rhizobial glb genes either by using the search tool of MS Word ® or by pairwise sequence alignments using the ClustalX program (http://www.clustal.org/clustal2/).org/clustal2/
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