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In total, 21 genes were identified and validated by sequence alignments, giving an overall gene density of about one gene per 7.8 kb, considering the partial gene (g1) that covers the 5' part of the BAC insert (Table 1; Figure 2).
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The original multiple sequence alignment gives rise to a distance matrix D. Each replicate dataset created by sampling columns with replacement from the alignment also gives its corresponding matrix B of perturbed pairwise distances.
The primers were designed in silico by sequence alignments based on available DNA sequence data.
This allows the user to retrieve proteins from Cildb, either in the whole database or for a given species, by sequence alignment using the NCBI BLAST algorithm.
Briefly, PhyloPhlAn locates 400 ubiquitous bacterial genes in a given assembly by sequence alignment in amino acid space, then builds a tree by concatenating the most discriminative positions in each gene into a single long sequence and applying FastTree (Price et al., 2010), a standard tree reconstruction tool.
A possible application would be to take intron positions into account when performing sequence alignments by giving a higher score if intron positions are conserved.
shRNAs were identified by sequence alignment using the Blat algorithm35.
Reads, averaging approximately 250 bases, were mapped on to the human reference genome, NCBI build 36, by sequence alignment using Basic Local Alignment Search Tool (BLAST -like alignment tool (BLAST -like
Sequence alignment of those highly similar sequences was confirmed by sequence alignment programs (ClustalW [ 42]).
The rearranged sequences cannot be easily detected by sequence alignment.
Additionally, multiple sequence alignment was also performed by sequence alignment packages inbuilt in MEGA 5.10 [ 48].
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