Sentence examples for by separation through from inspiring English sources

Over their millenary history and given the great distances between their communities, strengthened by separation through geographical barriers, warfare, and their political system of independent city-states, it may be expected that the Maya would have diverged into several distinct populations.

Fully mature gametocytes were harvested 17 days after initiation of the asexual culture, and purified by magnetic separation through a MACS column as described previously [35].

A control unadducted 73-mer template DNA and BPDE-adducted 11-mers (C10 S or C10 R on N adenine or N guanine) were purified by electrophoretic separation through 10%and15%5% polyacrylamide sequencing gels, respectively.

Restriction digestion of the ITS2 product with the enzyme Msp I (recognition motif CCGG) followed by size separation through a 3% agarose gel revealed eight RFLP profiles or genotypes with bands ranging from 50 bp to 350 bp.

RNA quality was determined by separation of the RNA through capillary electrophoresis using the Agilent 2000 Bioanalyzer (Agilent, Santa Clara, CA, USA).

Contraction of the abdominal wall causes laterally directed tension on the closure line and might cause the suture material to cut through by separation of the transversely orientated fibers [ 40, 41].

Molecular genetic approaches have been developed for analysis of TCR repertoires that involve amplification of cDNA using C and V specific primers, or genomic DNA between V and J specific primers, followed by separation of PCR products through denaturing gel electrophoresis.

Purified untouched CD4+ T cells (5×104 cells/well) or purified untouched CD8+ T cells (3×104 cells/well) were cultured on a feeder of spleen cells recovered from naïve unvaccinated mice and depleted of CD3+ T lymphocyte by a magnetic separation through a PE-isolation kit (Miltenyi Biotec) (1.5×105 cells/well).

Single cell suspensions were prepared by manual disruption and separation through a 70 µm cell strainer.

Single cell suspensions of splenocytes were prepared by mechanical disruption and separation through a 70-μm nylon cell strainer (BD Biosciences, Franklin Lakes, NJ, USA).

SMARTA transgenic CD4+ T cells were obtained by processing whole spleens through 70-μm nylon cell strainers followed by separation with anti-CD4 MicroBeads.