Sentence examples for by separation on a from inspiring English sources

Further purification of GAG species was obtained by separation on a 0.7×2.5cm Q-Sepharose HiTrap anion exchange column (GE Healthcare).

Human peripheral blood mononuclear cells (PBMC) were isolated from buffy coats obtained from healthy donors by separation on a Lymphoprep gradient (Nycomed, Oslo, Norway) according to standard procedures.

Immunoglobulin-coated LVP were then purified by incubating the low density fraction with protein A-coated microbeads (Miltenyi Biotec, Paris, France) and by separation on a magnetic column (Miltenyi Biotec).

Protein samples prepared were subjected to SDS-polyacrylamide gel electrophoresis by separation on a 12 15% gel, transferred to 0.2 µm Protran nitrocellulose membranes (PerkinElmer, Boston, MA), blocked in 5% dried milk powder in 50 mM Tris-HCl, pH 7.5, 15 mM NaCl, 0.5% Tween-20 (TBST), washed briefly in TBST and incubated in primary antibody in TBST overnight at 4°C.

The DNA was quantified in a NanoDrop spectrometer and by separation on a 0.8%% agarose gel.

The RNA was assessed for integrity and quantified on a NanoDrop spectrometer and by separation on a 1.2 % agarose gel.

The insert size was determined by digesting the insert out of the vector using the restriction enzymes EcoRI and XhoI, followed by separation on an agarose gel.

Short-term oligomerization of isolated Twinkle variants in vitro was tested by crosslinking the protein with low concentrations of glutaraldehyde followed by separation on an SDS PAGE and western blotting.

We assessed the quantity and quality of the RNA spectrophometrically (E260, E260/E280 ratio, spectrum 220 320 nm; Biochrom, Cambridge, UK) and by separation on an Agilent 2100 Bioanalyzer (Palo Alto, CA, USA).

Peptides were initially enriched on a reversed phase liquid chromatography (RPLC) pre-column (2 cm, 5 μ – 100 Ǻ), followed by separation on an analytical column (11 cm, 3 μ – 100 Ǻ) made with magic AQ C18 material (Michrom Bioresources, Inc, Auburn, CA) packed in-house.

Three of the variants were genotyped with other platforms: rs4340 was genotyped by a PCR assay followed by separation on 2% agarose gel with ethidium bromide staining and rs28665122 and rs3216183 were genotyped with TaqMan (Applied Biosystems, Foster City, CA) [30], [44].