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The integrity of DNA was assessed by separation in a 0.7% agarose gel (Sigma Aldrich) and staining with SYBR Safe (Invitrogen).
CD11b+ cells were isolated by suspending them in MACS buffer and staining them with anti-CD11b microbeads (Miltenyi Biotec) followed by separation in a magnetic field using an MS column (Miltenyi Biotec).
For 2D-BNGE, 50 75 µg of mitochondrial protein was applied on a 5 13% gradient BN gel followed by separation in a 12.5% denaturing gel (Schagger & von Jagow, 1991).
The samples were then rotated slowly at 4°C in a refrigerator for 4 hours to allow equilibration, followed by separation in a centrifuge (model 5415D; Eppendorf) at 4°C at a maximum speed of 2897968 g for a minimum of 10 minutes.
Similar(56)
Amino acids were analyzed in a Shimadzu HPLC system by separation in an ion-exchange column and post-column reaction with ninhydrin.
Chromatin fragment size was assessed by separation in an agarose gel, and one of the chromatin aliquots was saved as input.
A depollution process by separation in fluidized bed has been studied.
A model of volatile organic compound condensation in dense fluidized bed was used to simulate a new depollution process by separation in fluidized bed.
The shock oscillation was caused by separation in the diffuser.
Interference between the data streams of different users is avoided by separation in frequency domain.
The species coexistence is probably facilitated primarily by separation in time.
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