The concentrations of L-citrulline in the respective watermelon juices were estimated by separating the juice amino acids by thin layer chromatography [ 10] and comparing the intensity of each juice's L-citrulline spot, after staining with ninhydrin, to those of authentic L-citrulline co-developed on the chromatogram at known concentrations.
Lipoic acid incorporation was assessed by separating the cell lysates on a 4 12% gradient SDS-PAGE gel followed by transfer to nitrocellulose membrane and analysis by autoradiography.
Samples for SEC analysis were prepared by separating humic acid- like molecules from the media.
We previously speculated that IMAC could be extended from protein purification to the purification of nucleic acids, by separating nucleotides and single-stranded DNA/RNA from double-stranded DNA.
It is also worth noting that, on the receptor, these two domains are not contiguous but are separated by 25 amino acids whereas only a 6 amino acid hydrophobic stretch separates the two noncontiguous contact-mimicking domains in the surrogate.
Such a dataset consisting of UniProtKB accession (one per line) followed by comma separated amino acid ranges (e.g., Q8WXI7, 1 10,000, and 10,001 20,000) can be investigated to see if there are more nsSNVs in specific regions of the proteins.
Urea complexation is a method used by researchers to separate fatty acids (FAs) based on their molecular structure.
A DNA element containing the half-site AGGTCA, a direct repeat of the half-site spaced with 0 5 nucleic acids (DR0-DR5) and palindrome repeat of the half-site not separated by nucleic acids (Pal0) were employed.
The two C2H2 ZF of KREPA3 are located at the 5' part of the ORF and widely separated by 108 amino acids, The ZF mutants were constructed by replacing the two cysteines with alanines in each or both ZFs as previously described [49].
There are two Aspartates (D, D) separated by 90 amino acids, the second of which is separated from Glutamate (E) by 34 amino acid residues.
