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By separately testing the membrane and cytosolic fractions, we show that erythrocyte proteolytic activity is found only in the cytosol.
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The alteration in the metabolite profiles in response to light intensity was validated by a two-way ANOVA, separately testing the factors of cytoplasmic substitution, light treatment and their interaction (Additional file 3: Table S3 and Additional file 4: Table S4).
The substrate specificity was investigated by separately testing methanol, ethanol, 1-propanol, isobutanol, hexanol, isoamyl alcohol, glutamate, and α-ketoglutarate as substrates for the purified enzyme fraction.
We investigated scale-dependent effects of these anthropogenic rural activities on macroinvertebrates and physico-chemical variables by separately testing for effects of reach-scale disturbance and catchment-scale land use.
The allogeneic cross-over assay has been employed successfully to dissect these two possibilities by separately testing effector and Treg cells from a given donor to be suppressed or to suppress respectively a given population of Treg v effector cells from a healthy allogeneic donor [29] [31].
If necessary, variables were log-transformed to meet the assumptions of ANOVA: To visualise metabolic changes in the LC-FT-MS profiles of L. corniculatus in response to species diversity, cluster and variance analysis was combined with the TIGR MeV software version 4.1, whereby each metabolite is separately tested by the software.
Associations between ESBL type, co-resistance, mutations in ampC promoter regions, and phylogenetic groups were separately tested by using the χ or Fisher exact tests.
Following the guidelines proposed by Dimitrov (2010), we first analysed the configural invariance by testing the CFA baseline model separately for the two groups.
The Baz1b results were reproduced in two independent sets of pooled samples, and by testing the individual samples separately.
Both partitions were then separately tested for homoplasy by estimating the level of substitutional saturation.
The robust operation for parametric variation is ascertained by testing the controller with two separately excited DC motors with the same rating but different parameters.
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