Sentence examples for by semiquantitative reverse transcriptase from inspiring English sources

Methods: The authors measured intestinal cytokine mRNA expression by semiquantitative reverse transcriptase polymerase chain reaction in 21 infants with histologically confirmed NEC, 18 with other inflammatory conditions, and in 9 patients without intestinal inflammation.

The osteoblastic phenotype of the cells was confirmed by analysing the expression of bone-specific genes (Runx2, osteocalcin, osteopontin, and osteonectin) by semiquantitative reverse transcriptase polymerase chain reaction (PCR).

The BMPs PLAB, PDF and related protein INSL-4 were identified by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), but their mRNA expression was independent of gestational age (7 41 weeks of gestation).

Expression levels for receptors and ligands were assessed by semiquantitative reverse transcriptase polymerase chain reaction analysis.

The level of mRNA for MMP-2 and -9 was determined by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR).

A time-dependent increase in TfR1 mRNA was also detected by Leishmania major (Friedlin) infection into J774A.1 cells as found by semiquantitative reverse transcriptase-PCR (RT-PCR) (Fig. 2B).

On the other hand, we confirmed by semiquantitative reverse transcriptase-polymerase chain reaction that CYP1A1 is not expressed in CV-1 cells and that various PCB congeners do not induce CYP1A1 expression [Supplemental Material, Figure 1S (available online at http://www.ehponline.org/members/2008/11176/suppl.pdf)].

In 50 examined cases of gastric carcinoma, 4488%8%) cases showed a higher expression of TIMP-1 mRNA in the biopsy samples from the tumour tissue (T) than in the biopsy samples from the corresponding normal tissue (N), as determined by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR).

First, stable cell lines were verified for Id3 protein induction by Tet (Fig. 1A) and a robust induction of Id3 mRNA (seven- to eightfold) was confirmed by semiquantitative reverse transcriptase-mediated PCR (RT-PCR) as well as by qRT-PCR (Fig. S1).

The mRNA for ppET-1 and NOS III was determined by semiquantitative reverse-transcriptase polymerase chain reaction (RT-PCR).

Semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) studies were undertaken to define the expression pattern for D. virilis RNAs generated in the y ac intergenic region.