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Functional Ki-4 scFv were obtained by selection of binding phage on the Hodgkin lymphoma-derived, CD30-expressing cell line L540Cy.
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This approach might help reduce bias introduced by manual selection of binding site residues and introduces pocket shape information to the VL.
Affinity maturation was then achieved by random addition of residues flanking the minimal binding sequence and by selection of new α-bungarotoxin binding peptides on the basis of their dissociation kinetic rate.
Alkaline phosphatase conjugated magnetic microspheres were synthesized via amide reaction, and employed as an effective adsorbent in affinity selection of binding ligands followed by UHPLC-Q-TOF/MS analysis.
The experiments on extending a structural similarity measure can be divided into two lines of experiments: line "A" considers the by-hand selection of the binding relevant information that is used to extend the similarity measure and line "B" considers the data mining based selection of this information.
In addition to the above neutralizing epitopes identified by antibody binding assays, several immunodominant epitopes were identified by selection of escape mutants using neutralizing mAbs.
Hit expansion by selection of compounds from the Roche compound library identified tyramine derivatives with improved binding affinities as monitored by surface plasmon resonance.
Phage display results in linkage of an affinity-selectable function (the displayed peptide) to the DNA encoding that function, allowing selection of individual binding clones by interative cycles of in vitro panning and in vivo amplification.
The use of these staphylococcal surface display systems as alternatives for display of large protein libraries and subsequent affinity selection of relevant binding proteins by fluorescence-activated cell sorting (FACS) will be discussed.
Avidity enables the selection of filament binding over monomers, by a very large margin.
The negative selection of miRNA binding sites in coding regions was further supported by examining the mRNA expression profiles after miRNA misexpression.
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