The top nine candidates in the screen (Fig 1B, Supplementary Fig S1)—showing the highest repressed levels of normalized TCF-luficerase reporter activity above the baseline given by dnTCF were then selected and tested for inhibition of human Ls174T colon cancer cell proliferation, as this is WNT-TCF-dependent (van de Wetering et al, 2002).
Chloramphenicol- and kanamycin-resistant BAC-positive clones were selected and tested by PCR for homologous recombination by using two primer pairs P1/P2 and P3/P4 (see Fig. 1 and Materials and Methods) specific for the 5′- and 3′- recombination sites, respectively.
To verify this, seven DSTs were selected and tested by fourteen students enrolled in a graduate-level architecture design studio.
For this study a TiN hard coating manufactured by a PVD method was selected, and tested against a smooth polycrystalline alumina ball.
To estimate the seroprevalence of toxoplasmosis and risk factors for seropositivity in sheep, goats and cattle in Dhaka, Mymensingh, Sirajganj and Chittagong districts in Bangladesh, 1104 sera samples (552 sheep, 300 goats and 252 cattle) were randomly selected and tested by an indirect enzyme linked immunosorbent assay (iELISA).
A total of 89,832 unique putative SNP were identified from this pipeline and a sample of 48 SNP were randomly selected and tested by designing primers and sequencing a panel of 96 individuals on an ABI 3730xl sequencer.
‡Fifty IFA assay positive serum samples were randomly selected and tested by immunoblotting.
A subset of DEGs was randomly selected and tested by quantitative real-time PCR.
A total of 384 positive clones were selected and tested by PCR using vector primers T3/T7 and primer (AC 12.
Two hundred forty positive clones were selected and tested by PCR using (AG 10, (AC 10, and M13 universal primers, of which 197 clones contained potential microsatellite motifs.
