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The solution is carried out by selecting a sequence of direction vectors in the design space.
Therefore, in the design of the target sequence, the possibility of off-target activity was significantly reduced by selecting a sequence in which the next most related sequence in the Dictyostelium genome had at least 5 mismatches within 12 nucleotides of the PAM sequence.
Starting with a structure alignment, 1) S and T are created by selecting a sequence and simultaneously degapping both the sequence and structure.
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Fungal homologous sequences were identified using the BLASTP algorithm [ 65] with a cutoff E-value of 10-7 by randomly selecting a sequence from the database, finding its homologs, and removing the entire family from the database.
By selecting a subset of sequence clusters that fulfil alignment and homology criteria, both studies ended up with only 400-450 gene sets.
We found that targeting was highly efficient for every genomic target except LEU2, which at first was weak but was restored to 100% by selecting a different guide sequence (LEU2-2) as the sgRNA.
While the initial version of AdvISER-M-PYRO analyzed multiplex pyrosequencing signals by selecting a single and unique sequence for each genomic region, this new version enables the analysis of multiplex pyrosequencing signals generated from samples including two distinct sequences (i.e., plasmid and chromosomal) for each genomic region.
This was confirmed by selecting a few samples for sequencing.
The data used for detection of positive selection in gamma-glutamyl transpeptidase family was generated by selecting a subset of 23 DNA sequences from the earlier data set of 47 sequences.
When they were found to be lower than 30%, the 5F and 3R sequences and the three checking sequences were optimized with the software Primer3 (Primer3web version 4.0.0; http://primer3.ut.ee/) (Untergasser et al. 2012; Koressaar and Remm 2007) by re-selecting a sequence (between 24 and 35 bp) further upstream or downstream of the original site with at least 30% GC content.
The N1 and H5 trees of the HPAIV H5N1 clade were further reduced by randomly selecting a single sequence from all clades whose maximum distance from its basal node was less than 0.01.
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CEO of Professional Science Editing for Scientists @ prosciediting.com