Sentence examples for by seeds in coding from inspiring English sources
One problem in the interpretation of our results is that the detected enhancement of 3'UTR mediated regulation by seeds in coding region is just a correlation and therefore potentially the by-product of much more specific relationships between miRNA binding sites in coding regions and other known or unknown 3'UTR features.
The conservation of real and random miRNA seeds in coding region among 5 species was computed by using human, chimpanzee, mouse, rat and dog.
Thus, additional conserved 7mer/8mer seeds in coding region have more synergistic effect than conserved 6mer seeds on regulation of mRNAs.
We therefore normalized the seed number in coding regions by dividing the total number of seeds in coding regions by the length of the coding sequence but still were not able to find any clear correlation between normalized seed numbers and regulation.
We note that this synergistic effect of seeds in coding sequences is significant but weaker than the effect generated by additional 3'UTR seeds.
However, these studies analyzed seeds in coding regions independent of the existence of seeds in 3'UTRs.
To further understand the contribution of miRNA seeds in coding regions and their possible interaction with 3'UTR seeds, we analyzed the composite effect of miRNA seeds in coding regions and 3'UTRs by using large-scale, genome wide miRNA mis-expression data that were published recently by two laboratories [6], [7].
These groups were 1) mRNAs/proteins that have no seed in 3'UTRs or coding regions; 2) mRNAs/proteins that have seeds only in coding regions; 3) mRNAs/proteins that have one seed in 3'UTRs and no seed in coding regions; 4) mRNAs/proteins that have one seed in 3'UTRs and additional seeds in coding regions.
Altogether, the function of seeds in coding regions is not understood.
Furthermore, reporter assay experiments confirmed that miRNAs seeds in coding regions can be functional [10], [11].
This suggests that one possible function of seeds in coding regions is in helping recognition of seeds in 3'UTRs or in enhancing miRNA-target interactions.
